Mihaela Gropeanu, Maniraj Bhagawati, Radu A. Gropeanu, Gemma M. Rodríguez Muñiz, Subramanian Sundaram, Jacob Piehler and Aránzazu del Campo A Versatile Toolbox for Multiplexed Protein Micropatterning by Laser Lithography Small 9
Photocleavable oligohistidine peptides are synthesized for improved caging of tris-NTA-functionalized surfaces by multivalent interaction with complexed Ni(II) ions. Efficient photocleavage of these peptides by a confocal laser beam allows capturing of His-tagged proteins into microstructures under physiological conditions. By combination with an enzymatic transfer reaction, stable multiplexed protein arrays are generated.
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