Sébastien G. Ricoult, Mateu Pla-Roca, Roozbeh Safavieh, G. Monserratt Lopez-Ayon, Peter Grütter, Timothy E. Kennedy and David Juncker Large Dynamic Range Digital Nanodot Gradients of Biomolecules Made by Low-Cost Nanocontact Printing for Cell Haptotaxis Small 9
An easy, low cost, and massively parallel nanopatterning method based on lift-off nanocontact printing using disposable polymer masters is demonstrated. Its potential is shown by simultaneously patterning 64 digital nanodot gradients (DNGs) of proteins on a glass slide with a dynamic range of up to 4400 and comprising up to 419,790 200-nm-dots. Haptotaxis studies of C2C12 cells on DNGs of proteins and peptides validate DNGs for use in biological studies.
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