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Umit Hakan Yildiz, Hans-Peter M. De Hoog, Zhikang Fu, Nikodem Tomczak, Atul N. Parikh, Madhavan Nallani and Bo Liedberg Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments Small 10

Article first published online: 21 AUG 2013 | DOI: 10.1002/smll.201300060

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Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

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