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Umit Hakan Yildiz, Hans-Peter M. De Hoog, Zhikang Fu, Nikodem Tomczak, Atul N. Parikh, Madhavan Nallani and Bo Liedberg Polymersomes: Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments (Small 3/2014) Small 10

Version of Record online: 4 FEB 2014 | DOI: 10.1002/smll.201470019

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Polymersomes are encapsulated with a fluorescent reporter and a non-labeled enzyme for sensing of adenosine triphosphate (ATP). As B. Liedberg and co-workers report on page 442, passive diffusion of exogenously added ATP through the membrane is sensed by monitoring the ATPinduced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

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