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Sandra Scheele, Dan Oertel, Johannes Bongaerts, Stefan Evers, Hendrik Hellmuth, Karl-Heinz Maurer, Michael Bott and Roland Freudl Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum Microbial Biotechnology 6

Version of Record online: 20 NOV 2012 | DOI: 10.1111/1751-7915.12005

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In this study, we describe the successful secretion of a heterologous FAD cofactor-containing enzyme into the supernatant of Corynebacterium glutamicum by using the twin-arginine translocation (Tat) protein export machinery for protein translocation across the cytoplasmic membrane. Our results show for the first time that, also for cofactor-containing proteins, a secretory production strategy is a feasible and promising option.

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