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Miroslav Pátek, Jiří Holátko, Tobias Busche, Jörn Kalinowski and Jan Nešvera Corynebacterium glutamicum promoters: a practical approach Microbial Biotechnology 6

Version of Record online: 10 JAN 2013 | DOI: 10.1111/1751-7915.12019

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In this minireview, the main aspects of studies of promoters, the methods developed for their analysis and their practical use in Corynebacterium glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C. glutamicum promoter studies are outlined.

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