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Thiago L. P. Castro, Nubia Seyffert, Rommel T. J. Ramos, Silvanira Barbosa, Rodrigo D. O. Carvalho, Anne Cybelle Pinto, Adriana Ribeiro Carneiro, Wanderson Marques Silva, Luis G. C. Pacheco, Christopher Downson, Maria P. C. Schneider, Anderson Miyoshi, Vasco Azevedo and Artur Silva Ion Torrent-based transcriptional assessment of a Corynebacterium pseudotuberculosis equi strain reveals denaturing high-performance liquid chromatography a promising rRNA depletion method Microbial Biotechnology 6

Article first published online: 15 JAN 2013 | DOI: 10.1111/1751-7915.12020

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We hereby demonstrate an alternative rRNA depletion method which was tested in order to assess the transcriptional activity of a Corynebacterium pseudotuberculosis biovar equi strain using the Ion Torrent sequencing technology. This method, based on denaturing high-performance liquid chromatography (DHPLC), showed to subtract ribosomal transcripts in levels which resemble those seen when a commercial depletion kit was used. The DHPLC-based depletion method could be possibly employed to treat total RNA of other organisms and represent a cost-effective option when a suitable chromatographic system is available.

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