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Raweewan Thiramanas, Kulachart Jangpatarapongsa, Udom Asawapirom, Pramuan Tangboriboonrat and Duangporn Polpanich Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection Microbial Biotechnology 6

Article first published online: 9 JAN 2013 | DOI: 10.1111/1751-7915.12021

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Polystyrene (PS) nanoparticle copolymerized with acrylic acid (AA) and colored monomer (ALN8T) (PS/AA-ALN8T) was synthesized and used as a solid support of antibody specific to Plasmodium falciparum for malaria detection. Antibody immobilization was performed by three processes to compare the sensitivity and specificity. High sensitivity of 100% was obtained in all kinds of antibody coated PS/AA-ALN8T nanoparticles when tested with P. falciparum, while the antibody coated nanoparticles prepared from streptavidin-biotin interaction exhibited the highest specificity to P. falciparum.

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