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Encarna Garrido-Bailón, Mariano Higes, Amparo Martínez-Salvador, Karina Antúnez, Cristina Botías, Aránzazu Meana, Lourdes Prieto and Raquel Martín-Hernández The prevalence of the honeybee brood pathogens Ascosphaera apis, Paenibacillus larvae and Melissococcus plutonius in Spanish apiaries determined with a new multiplex PCR assay Microbial Biotechnology 6

Article first published online: 6 AUG 2013 | DOI: 10.1111/1751-7915.12070

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A multiplex polymerase chain reaction (PCR) assay was developed to simultaneously amplify the16S rRNA gene of Paenibacillus larvae and Melissococus plutonius, and the 5.8S rRNA gene of Ascosphaera apis, the main honey bee brood pathogens. The results of our transverse study revealed a low prevalence of honeybee brood pathogens in Spain in 2006 and 2007. The detection of infectious pathogens in honey bee brood samples without symptoms clearly demonstrates that they are not exclusively present in larvae that exhibit clinical signs of infection.

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