E-mail

E-mail a Wiley Online Library Link

Daniel Scheps, Sumire Honda Malca, Sven M. Richter, Karoline Marisch, Bettina M. Nestl and Bernhard Hauer Synthesis of ω-hydroxy dodecanoic acid based on an engineered CYP153A fusion construct Microbial Biotechnology 6

Article first published online: 14 AUG 2013 | DOI: 10.1111/1751-7915.12073

Thumbnail image of graphical abstract

We reported the construction of a catalytically active self-sufficient fusion protein (CYP153AM. aq.-CPRBM3) for efficient electron coupling and optimal expression in E. coli. The established biocatalytic system was used for the production of selective ω-hydroxylation of dodecanoic acid in shaking flasks. In a two-phase bioreactor-system using dodecanoic acid methyl ester as substrate and the additionally expressed transporter (AlkL) 4 g/L ω-hydroxy dodecanoic acid could be produced with non-engineered E. coli resting cells.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field

SEARCH