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Daniel Scheps, Sumire Honda Malca, Sven M. Richter, Karoline Marisch, Bettina M. Nestl and Bernhard Hauer Synthesis of ω-hydroxy dodecanoic acid based on an engineered CYP153A fusion construct Microbial Biotechnology 6

Version of Record online: 14 AUG 2013 | DOI: 10.1111/1751-7915.12073

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We reported the construction of a catalytically active self-sufficient fusion protein (CYP153AM. aq.-CPRBM3) for efficient electron coupling and optimal expression in E. coli. The established biocatalytic system was used for the production of selective ω-hydroxylation of dodecanoic acid in shaking flasks. In a two-phase bioreactor-system using dodecanoic acid methyl ester as substrate and the additionally expressed transporter (AlkL) 4 g/L ω-hydroxy dodecanoic acid could be produced with non-engineered E. coli resting cells.

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