Mingfeng Cao, Weitao Geng, Wei Zhang, Jibin Sun, Shufang Wang, Jun Feng, Ping Zheng, Anna Jiang and Cunjiang Song Engineering of recombinant Escherichia coli cells co-expressing poly-γ-glutamic acid (γ-PGA) synthetase and glutamate racemase for differential yielding of γ-PGA Microbial Biotechnology 6
The pgsBCA and racE genes from different original Bacillus strains had been firstly cloned and expressed in E. coli strains. It showed that LL3 derived PgsBCA had higher catalytic activity and enhanced productivity than NK-03 and the supply of L-glutamate could be served as the ascendance upon γ-PGA production. RacE integration could enhance γ-PGA yield through improving the preferred D-glutamate content.
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