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Rachel Morris, Anne Schauer-Gimenez, Ujwal Bhattad, Colleen Kearney, Craig A. Struble, Daniel Zitomer and James S. Maki Methyl coenzyme M reductase (mcrA) gene abundance correlates with activity measurements of methanogenic H2/CO2-enriched anaerobic biomass Microbial Biotechnology 7

Article first published online: 31 OCT 2013 | DOI: 10.1111/1751-7915.12094

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Using clone libraries and qPCR, we compared the diversity and abundance of mcrA genes and transcripts in four different methanogenic hydrogen/CO2 enrichment cultures to function, as measured by specific methanogenic activity assays (SMA) using H2/CO2. The mcrA gene copy number significantly correlated with CH4 production rates using H2/CO2 while correlations between mcrA transcript number and SMA were not significant.

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