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Zhao-Yuan Chen, Jie Cao, Li Xie, Xiao-Fei Li, Zhen-Hai Yu and Wang-Yu Tong Construction of leaky strains and extracellular production of exogenous proteins in recombinant Escherichia coli Microbial Biotechnology 7

Article first published online: 30 APR 2014 | DOI: 10.1111/1751-7915.12127

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Nine genetically stable leaky mutants were obtained. The leaky levels of Trx-hPTH in TB medium are higher than those in MR medium.The highest level of extracellular production of Trx-hPTH in fermenters is up to 680 mg/l

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