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J. Verrier, L. Krähenbühl, O. Bontems, M. Fratti, K. Salamin and M. Monod Dermatophyte identification in skin and hair samples using a simple and reliable nested polymerase chain reaction assay British Journal of Dermatology 168

Version of Record online: 30 JAN 2013 | DOI: 10.1111/bjd.12015

What’s already known about this topic?

  •  Polymerase chain reaction (PCR) sequencing assays have been developed for the direct identification of dermatophytes from dermatological samples but without distinction between onychomycosis and other tinea.
  •  The failure to isolate a dermatophyte in cultures frequently occurs, especially in cases of previous antifungal therapy.
  •  The problem with direct PCR dermatophyte identification in skin and hair differs from that in onychomycosis because a small amount of material is generally collected from patients for mycological analysis.

What does this study add?

  •  A nested PCR assay was found to be necessary for sensitive sequencing and dermatophyte identification in tinea capitis and tinea corporis.
  •  Improved sensitivity of dermatophyte identification was obtained as it was possible to identify the dermatophyte when the fungus failed to grow in cultures.
  •  This assay is especially suitable for tinea capitis as the appropriate treatment depends on the incriminated dermatophyte species.

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