Ji-Yoen Kim, Ryan T. Ash, Carolina Ceballos-Diaz, Yona Levites, Todd E. Golde, Stelios M. Smirnakis and Joanna L. Jankowsky Viral transduction of the neonatal brain delivers controllable genetic mosaicism for visualising and manipulating neuronal circuits in vivo European Journal of Neuroscience 37
Freehand injection of recombinant AAV into the neonatal mouse brain offers a fast and easy way to attain widespread genetic manipulation of neurons throughout the brain. Rapid onset and year-long persistence of viral expression permits study of both critical periods and aging. Viral titer can be used to control mosaicism, and multiple viruses can be co-injected for bigenic expression. The technique's simplicity and the availability of viral reagents should facilitate a range of experiments.
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