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Ken Ichi Matsuda, Miho Yanagisawa, Kazuhiro Sano, Ikuo Ochiai, Sergei Musatov, Kota Okoshi, Shinji Tsukahara, Sonoko Ogawa and Mitsuhiro Kawata Visualisation and characterisation of oestrogen receptor α-positive neurons expressing green fluorescent protein under the control of the oestrogen receptor α promoter European Journal of Neuroscience 38

Version of Record online: 19 APR 2013 | DOI: 10.1111/ejn.12227

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Transgenic mice expressing GFP under the control of the ERα promoter has been generated. GFP-positive neurons were observed in areas previously reported to express ERα mRNA, and GFP signals mostly overlapped with ERα immunoreactivity. Targeting of ERα gene by adeno-associated virus-mediated small hairpin RNA expression induced GFP in the medial preoptic area, suggesting a negative feedback mechanism in which ERα controls expression of the ERα gene itself.

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