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Tina Notter, Patrizia Panzanelli, Sandra Pfister, Dennis Mircsof and Jean-Marc Fritschy A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system European Journal of Neuroscience 39

Article first published online: 10 DEC 2013 | DOI: 10.1111/ejn.12447

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This technical spotlight describes a tissue preparation protocol suitable for performing biochemical experiments and immunohistochemistry under optimal conditions with CNS tissue from the same mouse. We illustrate the versatility of this protocol with examples of Western blotting, real-time PCR, immunoperoxidase staining, immuno-electron microscopy, and high sensitivity immunofluorescence staining in various mouse brain tissue preparations.

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