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Rodolfo García-Contreras, Paul Vos, Hans V. Westerhoff and Fred C. Boogerd Why in vivo may not equal in vitro – new effectors revealed by measurement of enzymatic activities under the same in vivo-like assay conditions The FEBS Journal 279

Version of Record online: 12 OCT 2012 | DOI: 10.1111/febs.12007

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Does the understanding of the dynamics of biochemical networks in vivo, in terms of the properties of their components determined in vitro, require the latter to be determined under the same conditions? An in vivo-like assay medium for enzyme activity determination was designed based on the concentrations of the major ionic constituents of the E. coli cytosol.

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