Anna Fryszkowska, Helen S. Toogood, David Mansell, Gill Stephens, John M. Gardiner and Nigel S. Scrutton A surprising observation that oxygen can affect the product enantiopurity of an enzyme-catalysed reaction The FEBS Journal 279
Pentaerythritol tetranitrate reductase catalyses the NAD(P)H-dependent reduction of (E)-2-phenyl-1-nitroprop-2-ene. Under aerobic conditions, it undergoes FMNred reoxidation by oxygen, generating hydrogen peroxide and superoxide (ROS). The presence of oxygen and ROS influenced 2-phenyl-1-nitropropane product enantiopurity, by increasing the proportion of (R)- and (S)-alkane generated, respectively. This may be due to the presence of oxidatively modified cysteine and/or methionine residues.
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