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Nageswara R. Alla and Allen W. Nicholson Evidence for a dual functional role of a conserved histidine in RNA·DNA heteroduplex cleavage by human RNase H1 The FEBS Journal 279

Version of Record online: 9 NOV 2012 | DOI: 10.1111/febs.12035

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The conserved enzyme RNase H1 is the catalytic agent in gene silencing by oligodeoxynucleotide antisense drugs. An optimized in ;vitro assay involving purified recombinant human RNase H1 can recapitulate the in vivo pathway of antisense oligonucleotide-directed RNA cleavage, and is used to show that the highly conserved histidine 264 is important for both the hydrolytic and product release steps

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