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Massimo Sabbatini, Alessandro Vezzoli, Mario Milani and Giovanni Bertoni Evidence for self-association of the alternative sigma factor σ54 FEBS Journal 280

Article first published online: 11 FEB 2013 | DOI: 10.1111/febs.12129

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σ54 was thought to act as monomer in all transcription steps. We provide evidence that σ54 of Pseudomonas putida interacts with itself. The self-self interface amid monomers involves contacts in the σ54 regions I and III. We propose a model of σ54 self-association in tetramers envisaged as “dimer of dimers”. Our results suggest a new auto-antagonistic regulatory state of σ54

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