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Xinrui Zhou, Kathleen Szeker, Bernd Janocha, Thomas Böhme, Dirk Albrecht, Igor A. Mikhailopulo and Peter Neubauer Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides The FEBS Journal 280

Version of Record online: 21 FEB 2013 | DOI: 10.1111/febs.12143

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Recombinant purine nucleoside phosphorylases from Deinococcus geothermalis, Geobacillus thermoglucosidasius and Aeropyrum pernix geothermalis were prepared and their thermostability, kinetic properties, substrate specificities towards (deoxy)adenosine, (deoxy)inosine, (deoxy)cytidine and C2′-modified (aminodeoxy, deoxyfluoro) nucleosides investigated. DgPNP and ApMTAP are promising biocatalysts in the synthesis of 9-(2-deoxy-2-fluoro-⁢-D-ribo/arabino-furanosyl)adenines using the corresponding uracil deoxyfluoro nucleosides as the pentofuranose donors in the transglycosylation reaction of adenine

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