Natacha Dreumont and Bertrand Séraphin Rapid screening of yeast mutants with reporters identifies new splicing phenotypes The FEBS Journal 280
Mutations of several spliceosome-associated proteins from yeast do not affect pre-mRNA splicing. We show that splicing defects can be detected in many such mutants by using a set of pre-mRNA reporters with a wide range of splicing characteristics. For example, deletion of a proline-rich region at the N-terminus of Prp8 induces alternative usage of a duplicated branchpoint/3′ splice site region.
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