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Takatsugu Miyazaki, Megumi Ichikawa, Gaku Yokoi, Motomitsu Kitaoka, Haruhide Mori, Yoshikazu Kitano, Atsushi Nishikawa and Takashi Tonozuka Structure of a bacterial glycoside hydrolase family 63 enzyme in complex with its glycosynthase product, and insights into the substrate specificity The FEBS Journal 280

Version of Record online: 26 JUL 2013 | DOI: 10.1111/febs.12424

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We converted a glycoside hydrolase family 63 enzyme, Escherichia coli YgjK, to a glycosynthase to probe its substrate specificity. The reactions with β-d-glucosyl fluoride and monosaccharides showed that the largest amount of glycosynthase product accumulated when galactose was employed as an acceptor molecule. The crystal structure of E727A indicated that the disaccharide bound at the active site was 2-O-α-d-glucopyranosyl-d-galactopyranose.

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