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H. Thomason and D. J. S. Montagnes Developing a quick and inexpensive in vitro (non-animal) bioassay for mascara irritation International Journal of Cosmetic Science 36

Article first published online: 3 DEC 2013 | DOI: 10.1111/ics.12106

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The two model ciliated protozoa used in this study: (A) Blepharisma japonicum and (B) Paramecium caudatum. The genus Blepharisma is named after the long, ciliated feeding structure illustrated here that resembles an eyelash (Gk. blepharidos), whereas Paramecium, based on its shape, was originally, and still is, affectionately described as the ‘slipper ciliate’. Both are large, easily recognizable, slow-moving ciliates that lend themselves to bioassay work where cells are directly counted by microscopy. The pink pigmentation of B. japonicum allows it to be easily seen under the microscope and even as ‘pink specks’ when view with the naked eye. Scale bar = 100 μm. The photograph of Blepharisma japonicum is courtesy of Frank Fox, www.elektrotechnik.fh-trier.de; the photograph of Paramecium caudatum is courtesy of the Protist Information Server, URL: http://protist.i.hosei.ac.jp/PDB5/PCD0112/D/93.jpg

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