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Nitat Sinsereekul, Tita Foophow, Mai Yamanouchi, Yuichi Koga, Kazufumi Takano and Shigenori Kanaya An alternative mature form of subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis identified in the presence of Ca2+ The FEBS Journal 278

Version of Record online: 13 APR 2011 | DOI: 10.1111/j.1742-4658.2011.08107.x

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A subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis is thought to be matured from its inactive precursor Pro-Tk-SP upon autoprocessing of the N-and C-propeptides. In this study, however, we showed that the Pro-Tk-SP derivative with the N-propeptide removed, ProC-Tk-SP, represents a mature form of Pro-Tk-SP in a natural environment. The C-propeptide contributes to the stabilization of ProC-Tk-SP.

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