Majety Naga Leelaram, Anuradha Gopal Bhat, Shivanand Manjunath Hegde, Ramanathapuram Manjunath and Valakunja Nagaraja Inhibition of type IA topoisomerase by a monoclonal antibody through perturbation of DNA cleavage–religation equilibrium The FEBS Journal 279
Type IA DNA topoisomerases from bacteria are potential drug targets for which inhibitors are being discovered now. We identified a monoclonal antibody, 2F3G4, which specifically inhibited mycobacterial topoisomerase I in nanomolar range. The antibody stimulated enzyme-mediated DNA cleavage and elevated protein-DNA covalent adducts. The mode of inhibition opens up avenues to develop new, small molecule inhibitors for bacterial topoisomerase I.
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