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Emma J. Murphy, Clive L. Metcalfe, Chukwudi Nnamchi, Peter C. E. Moody and Emma L. Raven Crystal structure of guaiacol and phenol bound to a heme peroxidase FEBS Journal 279

Article first published online: 5 DEC 2011 | DOI: 10.1111/j.1742-4658.2011.08425.x

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Guaiacol is a universal substrate for all peroxidases and its use in a simple colorimetric assay has wide applications. However its exact binding location has never been defined. Here we report the crystal structures of guaiacol bound to cytochrome c peroxidase (CcP). A related structure, with phenol bound, is also presented.

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