Kwon-Young Choi, EunOk Jung, Da-Hye Jung, Bishnu Prasad Pandey, Hyungdon Yun, Hyung-yun Park, Romas J. Kazlauskas and Byung-Gee Kim Cloning, expression and characterization of CYP102D1, a self-sufficient P450 monooxygenase from Streptomyces avermitilis The FEBS Journal 279
CYP102D1 from S. avermitilis is a naturally self-sufficient CYP. Codon-optimized synthetic CYP102D1 was functionally active and catalyzed long chain fatty acid hydroxylation similar to other CYP102 family. We constructed a homology model of CYP102D1 in order to alter substrate specificity to aromatic compounds revealing that replacement of F96V/M246I increased catalytic activity 15 times higher for daidzein.
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