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Jiyong Su and Karl Forchhammer Determinants for substrate specificity of the bacterial PP2C protein phosphatase tPphA from Thermosynechococcus elongatus FEBS Journal 280

Article first published online: 23 JAN 2012 | DOI: 10.1111/j.1742-4658.2011.08466.x

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The mechanism of specific substrate recognition by Mg2+/Mn2+-dependent protein phosphatases (PPM/PP2C) is unexplained so far. Novel mechanistic insight in this issue was gained by site-directed mutagenesis of the cyanobacterial PPM-phosphatase tPphA (specific for signalling protein PII). PII-dephosphorylation requires fine-tuned protein interactions over the catalytic face of tPphA, including the highly conserved His39 residue and the flap subdomain, in addition to the catalytic centre.

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