Jiyong Su and Karl Forchhammer Determinants for substrate specificity of the bacterial PP2C protein phosphatase tPphA from Thermosynechococcus elongatus The FEBS Journal 280
The mechanism of specific substrate recognition by Mg2+/Mn2+-dependent protein phosphatases (PPM/PP2C) is unexplained so far. Novel mechanistic insight in this issue was gained by site-directed mutagenesis of the cyanobacterial PPM-phosphatase tPphA (specific for signalling protein PII). PII-dephosphorylation requires fine-tuned protein interactions over the catalytic face of tPphA, including the highly conserved His39 residue and the flap subdomain, in addition to the catalytic centre.
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