Ashley J. Warman, Jacob W. Robinson, Dominika Luciakova, Andrew D. Lawrence, Ker R. Marshall, Martin J. Warren, Myles R. Cheesman, Stephen E. J. Rigby, Andrew W. Munro and Kirsty J. McLean Characterization of Cupriavidus metallidurans CYP116B1 – A thiocarbamate herbicide oxygenating P450–phthalate dioxygenase reductase fusion protein The FEBS Journal 279
Article first published online: 21 MAR 2012 | DOI: 10.1111/j.1742-4658.2012.08543.x
The novel P450-phthalate dioxygenase reductase fusion enzyme CYP116B1 from Cupriavidus metallidurans was expressed/purified and shown to bind heme, FMN and 2Fe-2S cofactors. CYP116B1 is a monomeric protein that uses NAD(P)H to provide electrons for P450 catalysis. NADPH is preferred, reducing the CYP116B1 reductase at > 70 s−1. CYP116B1 catalyses hydroxylation and N-dealkylation reactions, to break down the thiocarbamate herbicides vernolate/EPTC.
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