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Jose-Luis Sagripanti, Gudrun Grote, Bärbel Niederwöhrmeier, Birgit Hülseweh and Hans-Jürgen Marschall Photochemical Inactivation of Pseudomonas aeruginosa Photochemistry and Photobiology 88

Article first published online: 22 NOV 2011 | DOI: 10.1111/j.1751-1097.2011.01029.x

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Adaptability to a broad range of environments together with relatively high resistance to antibiotics and disinfectants makes Pseudomonas aeruginosa a concern in hospitals and in public health. Photoinactivation with either INA or AMO at conditions that abolished bacterial infectivity did not impair PCR and ELISA testing. For comparison, similar titers of Bacillus atrophaeus spores (a surrogate for B. anthracis) remained unaffected at conditions that reduced the survival of P. aeruginosa below detection levels. The results presented in this study should assist in improved inactivation methods of environmental, clinical and forensic samples without impairing subsequent nucleic acid- or immune-based analysis.

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