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Sónia Vieira, Lourenço Ribeiro, Bruno Jesus, Paulo Cartaxana and Jorge Marques da Silva Photosynthesis Assessment in Microphytobenthos Using Conventional and Imaging Pulse Amplitude Modulation Fluorometry Photochemistry and Photobiology 89

Article first published online: 13 SEP 2012 | DOI: 10.1111/j.1751-1097.2012.01224.x

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Image of minimum chlorophyll a fluorescence of a dark-adapted sample during a diurnal low tide for mud (A) and sand (B) microphytobenthos. Imaging pulse amplitude modulated (Imaging-PAM chlorophyll fluorometry is a breakthrough in the study of spatial heterogeneity of different photosynthetic assemblages. However, Imaging-PAM uses different technology than conventional PAM, making comparisons between these techniques doubtful. Thereby, photosynthetic processes were comparatively assessed using conventional (Junior PAM and PAM 101) and Imaging-PAM on intertidal MPB (mud and sand) and on cork oak leaves. Our findings emphasize the caution needed when interpreting chlorophyll fluorescence data of MPB communities.

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