John A. Kyndt, Terry E. Meyer, Kenneth T. Olson, Jozef Van Beeumen and Michael A. Cusanovich Photokinetic, Biochemical and Structural Features of Chimeric Photoactive Yellow Protein Constructs Photochemistry and Photobiology 89
Article first published online: 9 OCT 2012 | DOI: 10.1111/j.1751-1097.2012.01235.x
Three chimeras of PYP were constructed that have Rhodobacter capsulatus PYP regions exchanged in a Halorhodospira halophila PYP backbone. With more regions exchanged, we observed decreased overall protein stability and increased solvent exposure in the ground state, while the spectra showed increasing amounts of the intermediate spectral form (λmax = 380 nm). Chimeras that have the β4–β5 loop exchange show at least 20 000 times slower recovery kinetics as compared to the prototypical H. halophila PYP.
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