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Eva Chamorro, Cristina Bonnin-Arias, María Jesús Pérez-Carrasco, Javier Muñoz de Luna, Daniel Vázquez and Celia Sánchez-Ramos Effects of Light-emitting Diode Radiations on Human Retinal Pigment Epithelial Cells In Vitro Photochemistry and Photobiology 89

Article first published online: 9 OCT 2012 | DOI: 10.1111/j.1751-1097.2012.01237.x

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Effects of monochromatic and white LED lighting on human retinal pigment epithelial cells in vitro. HRPEpiC cells were exposed to LED lighting (irradiated cells) or maintained in darkness (control) for three light–darkness cycles (12 h/12 h). Apoptosis determined by the activation of caspases-3, -7 is observed as a pink coloration around DAPI-stained cells: (1) Representative images of effects of LED lighting on HRPEpiC cells and (2) Graphs displays mean fluorescence intensity radios of irradiated cells versus non irradiated controls. Bars represent mean ± SD from = 3–5 experiments. The asterisk (*) indicates significant differences as compared to controls (< 0.05, Student's t-test).

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