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Raymond J. Ritchie and John W. Runcie Photosynthetic Electron Transport in an Anoxygenic Photosynthetic Bacterium Afifella (Rhodopseudomonas) marina Measured Using PAM Fluorometry Photochemistry and Photobiology 89

Article first published online: 19 OCT 2012 | DOI: 10.1111/j.1751-1097.2012.01241.x

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Readily available blue-diode based pulse amplitude modulation (PAM) fluorimeters can be used to measure photosynthetic electron transport (ETR) in nonsulfur purple photosynthetic bacteria. PAM machines can measure photosynthesis not only in oxygenic photorganisms and in those with a RC-2 photosystem. Figure shows a comparison of the electron transport rate (ETR) vs irradiance of acetate-grown Afifella cells with and without added acetate (5 mol m−3). ETR in both cases show a waiting-in-line saturation curve with inhibition at high irradiances (r > 0.9731). Added acetate increases ETR but increases optimum irradiance as well.

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