Boris Litsanov, Melanie Brocker and Michael Bott Glycerol as a substrate for aerobic succinate production in minimal medium with Corynebacterium glutamicum Microbial Biotechnology 6
Article first published online: 18 APR 2012 | DOI: 10.1111/j.1751-7915.2012.00347.x
This manuscript demonstrates the ability of a metabolically engineered strain of Corynebacterium glutamicum to produce succinate from glycerol, a by-product of biodiesel production, under aerobic conditions. Succinate made microbially from renewable carbon sources can serve as a precursor for a variety of chemical bulk products and for polymers and therefore is considered as a future platform chemical. For glycerol conversion to succinate by C. glutamicum, the genes encoding succinate dehydrogenase and acetate-forming enzymes were deleted and the Escherichia coli genes glpFKD coding for glycerol facilitator, glycerol kinase and glycerol 3-phosphate dehydrogenase were introduced. Under batch conditions, the resulting strain formed 9.3 g/l succinate from 34.5 g/l glycerol.
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