Isamu Aiba, Adrian K. West, Christian T. Sheline and C. William Shuttleworth Intracellular dialysis disrupts Zn2+ dynamics and enables selective detection of Zn2+ influx in brain slice preparations Journal of Neurochemistry 125
Presynaptic Zn2+ release and post-synaptic accumulation is implicated in neuronal physiology and pathology; however, direct detection of Zn2+ translocation can be masked by intracellular buffering. This study exploited intracellular dialysis by whole-cell clamp to deplete intracellular Zn2+ buffers and promote detection of Zn2+ translocation. The approach described here should help understand Zn2+ homeostasis by assisting identification of Zn2+ source(s) contributing to Zn2+ signalling.
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