E-mail a Wiley Online Library Link

Hugo Vicente Miranda, Wei Xiang, Rita M. de Oliveira, Tânia Simões, José Pimentel, Jochen Klucken, Deborah Penque and Tiago F. Outeiro Heat-mediated enrichment of α-synuclein from cells and tissue for assessing post-translational modifications Journal of Neurochemistry 126

Version of Record online: 25 APR 2013 | DOI: 10.1111/jnc.12251

Thumbnail image of graphical abstract

In this study, we describe a method for enriching alpha-synuclein (α-syn) from a variety of biological samples, from cultured cells to brain tissues. Enrichment of α-syn was achieved by heating samples, further facilitating the identification of specific post-translational modifications by immunoblot, or mass spectrometry-based techniques. This approach will contribute to the clarification of the role of α-syn PTMs in Parkinson's disease.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field