Hugo Vicente Miranda, Wei Xiang, Rita M. de Oliveira, Tânia Simões, José Pimentel, Jochen Klucken, Deborah Penque and Tiago F. Outeiro Heat-mediated enrichment of α-synuclein from cells and tissue for assessing post-translational modifications Journal of Neurochemistry 126
In this study, we describe a method for enriching alpha-synuclein (α-syn) from a variety of biological samples, from cultured cells to brain tissues. Enrichment of α-syn was achieved by heating samples, further facilitating the identification of specific post-translational modifications by immunoblot, or mass spectrometry-based techniques. This approach will contribute to the clarification of the role of α-syn PTMs in Parkinson's disease.
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