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Nelli Mnatsakanyan and Michaela Jansen Experimental determination of the vertical alignment between the second and third transmembrane segments of muscle nicotinic acetylcholine receptors Journal of Neurochemistry 125

Article first published online: 30 APR 2013 | DOI: 10.1111/jnc.12260

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The vertical alignment between the channel-lining transmembrane segment M2 and segment M3 in Cys-loop ligand-gated ion channels is a topic of controversy. While the vertical alignments as observed in X-ray structures of prokaryotic Gloeobacter violaceus ligand-gated ion channel (GLIC) and eukaryotic Caenorhabditis elegans glutamate-gated chloride channel α (GluClα) are in agreement, the Torpedo nAChR model based on electronmicroscopy differs. We determined the register between M2 and M3 in muscle nAChR by disulfide crosslinking. Our results are best reflected if modeled on GluClα, but not if modeled on the current Torpedo nAChR model. Overall, this indicates that the vertical alignment in nAChR is conserved between prokaryotes and eukaryotes.

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