E-mail a Wiley Online Library Link

Oliver Holmes, Swetha Paturi, Michael S. Wolfe and Dennis J. Selkoe Functional analysis and purification of a Pen-2 fusion protein for γ-secretase structural studies Journal of Neurochemistry 131

Version of Record online: 18 JUN 2014 | DOI: 10.1111/jnc.12772

Thumbnail image of graphical abstract

We present a method by which an MBP-tagged form of the Pen-2 subunit of γ-secretase may be purified from bacteria to mg quantities at greater than 95% purity. We show that this construct is incorporated into and allows full activity of γ-secretase in a mammalian system. These methods provide valuable tools to study the structure and function of Pen-2 and γ-secretase.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field