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Oliver Holmes, Swetha Paturi, Michael S. Wolfe and Dennis J. Selkoe Functional analysis and purification of a Pen-2 fusion protein for γ-secretase structural studies Journal of Neurochemistry 131

Version of Record online: 18 JUN 2014 | DOI: 10.1111/jnc.12772

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We present a method by which an MBP-tagged form of the Pen-2 subunit of γ-secretase may be purified from bacteria to mg quantities at greater than 95% purity. We show that this construct is incorporated into and allows full activity of γ-secretase in a mammalian system. These methods provide valuable tools to study the structure and function of Pen-2 and γ-secretase.

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