N. Nakashima and T. Tamura Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline-inducible vectors Letters in Applied Microbiology 56
Significance and Impact of the Study: A gene silencing method using antisense RNAs in Escherichia coli is described, which facilitates the investigation of bacterial gene function. In particular, the method is suitable for comprehensive analyses or phenotypic analyses of genes essential for growth. Here, we describe expansion of vector variations for expressing antisense RNAs, allowing choice of a vector appropriate for the target genes or experimental purpose.
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