B. Setlow, J. Yu, Y.-Q. Li and P. Setlow Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite Letters in Applied Microbiology 57
Significance and Impact of the Study: This work shows that with Bacillus subtilis spore populations in which approximately 95% of individual spores were killed by several oxidizing agents, >95% of the spores in these populations germinated with nutrients, albeit slowly. This is important, as assay of an early germination event, release of dipicolinic acid, has been suggested as a rapid assay for spore viability and would give false-positive readings for the level of the killing of oxidizing agent-treated spore populations. Analysis of the germination kinetics of multiple individual untreated or oxidizing agent-treated spores also provides new information on proteins damaged by oxidizing agent treatment, and at least some of which are in spores' inner membrane.
Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf