GUILLAUME MARTI, MATTHIAS ERB, JULIEN BOCCARD, GAÉTAN GLAUSER, GWLADYS R. DOYEN, NEIL VILLARD, CHRISTELLE A M. ROBERT, TED C. J. TURLINGS, SERGE RUDAZ and JEAN-LUC WOLFENDER Metabolomics reveals herbivore-induced metabolites of resistance and susceptibility in maize leaves and roots Plant, Cell & Environment 36
In the presented manuscript, we developed an ultra-high pressure liquid chromatography time-of-flight mass spectrometry (UHPLC-TOF-MS) based metabolomics approach to study how maize plants respond to attack by the generalist herbivore Spodoptera littoralis. Using multivariate data analysis, microflow nuclear magnetic resonance and direct nano-infusion tandem MS/MS, we systematically extracted, identified, quantified and tested major metabolites that are differentially regulated in maize seedlings. With this approach, our investigation provides deep insights into the dynamic patterns of local and systemic metabolic regulation.
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