Ruby H. Chan, James W. Lewis and Roberto A. Bogomolni Photocycle of the LOV-STAS Protein from the Pathogen Listeria monocytogenes. Photochemistry and Photobiology 89
Listeria monocytogenes is a food-borne bacterial pathogen responsible for listeriosis, an infectious disease in the human host. The pathogen has been identified to contain the Listeria Lmo0799 protein, a virulence factor activated by blue light. Genomic search has identified a LOV-domain protein coupled to a STAS domain, homologues to Bacillus subtilis YtvA, to be the only light sensor so far. We have cloned, expressed in E. coli and purified the full-length LM-LOV-STAS. Here, we report the photocycle and other spectroscopic features of the protein. Although it is a homolog of YtvA, LM-LOV-STAS has unique structural and spectroscopic features that are not reported for YtvA.
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