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Faina Nakonechny, Anna Pinkus, Smadar Hai, Ortal Yehosha, Yeshayahu Nitzan and Marina Nisnevitch Eradication of Gram-Positive and Gram-Negative Bacteria by Photosensitizers Immobilized in Polystyrene Photochemistry and Photobiology 89

Version of Record online: 27 DEC 2012 | DOI: 10.1111/php.12022

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Immobilization of photosensitizers in polymers opens prospects for their continuous and reusable application. Methylene blue (MB) and Rose Bengal were immobilized in polystyrene by mixing solutions of the photosensitizers in chloroform with a polymer solution, followed by air evaporation of the solvent. This procedure yielded 15–140 μm polymer films with a porous surface structure. The method chosen for immobilization ensured 99% enclosure of the photosensitizer in the polymer. The antimicrobial activity of the immobilized photosensitizers was proven against Gram-positive and Gram-negative bacteria (Figure). Photosensitizers immobilized on a solid phase can be applied for continuous disinfection of bacteria in wastewater.

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