Richard Breia, Sónia Vieira, Jorge Marques da Silva, Hernâni Gerós and Ana Cunha Mapping Grape Berry Photosynthesis by Chlorophyll Fluorescence Imaging: The Effect of Saturating Pulse Intensity in Different Tissues Photochemistry and Photobiology 89
Imaging-PAM proved to be a sensitive technique for the study of in vivo photosynthesis in grape berry, allowing detecting tissue-specific differences. It was also found that in photosynthetic heterogeneous systems like grape berry sections, with tissues adapted to different light intensities, the optimization of the saturation pulse intensity is crucial for the accurate determination of photochemical parameters, as photoinhibition may occur at moderate light intensities and in a tissue-dependent manner. Being a fleshy fruit, with a large watery pericarp limiting light and gas diffusion, it was interesting to find a photosynthetically active seed outer integument, suggesting a key role for seed photosynthesis.
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