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Sindhu G. Nair and Glen R. Loppnow Multiplexed, UVC-Induced, Sequence-Dependent DNA Damage Detection Photochemistry and Photobiology 89

Version of Record online: 22 MAR 2013 | DOI: 10.1111/php.12066

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A 96-well microplate is developed here as a platform for the detection of DNA damage in a number of DNA sequences simultaneously. This convenient mix-and-read assay uses smart probes, a hybridization assay composed of fluorescently labeled ssDNA hairpins complementary to the damaged target sequences, as general probes of DNA damage. The results show that the 96-well microplate platform is a simple, fast and inexpensive mix-and-read technique for multiplexed, sequence-specific DNA damage detection.

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