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Raymond J. Ritchie The Use of Solar Radiation by the Photosynthetic Bacterium, Rhodopseudomonas palustris: Model Simulation of Conditions Found in a Shallow Pond or a Flatbed Reactor Photochemistry and Photobiology 89

Version of Record online: 3 AUG 2013 | DOI: 10.1111/php.12124

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Blue-diode based pulse amplitude modulation (PAM) fluorimeters can be used to measure photosynthetic electron transport (ETR) in non-sulfur purple photosynthetic bacteria. Photosynthesis can be calculated on BChl a basis or in terms of volume of culture filtered onto a glass fibre disk. The figure shows a comparison of the electron transport rate (ETR) vs pond depth for Rhodopseudomonas palustris and Afifella marina cells with acetate + benzoate and acetate as carbon sources respectively. Both curves exhibit classical photoinhibition at shallow depths. Rhodopseudomonas achieves much higher total photosynthesis than Afifella because it is able to use high irradiances more effectively.

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